Reconstitution Guidelines

Reconstitution Guide

Learn how to reconstitute lyophilized (freeze-dried) semaglutide for research use

Semaglutide Reconstitution Steps

Ensure all reconstitution steps are performed under aseptic conditions to prevent contamination.
Use sterile vials, syringes, and needles throughout the process.
Determine the desired concentration of the peptide solution based on your experimental requirements.
Add bacteriostatic water to the semaglutide slowly and in small quantities to avoid loss of peptide during reconstitution.
Gently swirl the vial to dissolve the peptide. Avoid shaking, as it may cause foaming or denaturation.
Allow the lyophilized powder to dissolve fully. This may take some time depending on the peptide and its solubility.
If the peptide solution contains undissolved particles, consider using a sterile syringe filter to remove any particulate matter.

Semaglutide Concentration

To reach the desired semaglutide concentration you need to determine the volume of solvent (bacteriostatic water) you need to add to the semaglutide vial. To do that use the following formula:

The following are examples of how to calculate the volume of solvent to create specific concentrations in 2 mg and 5 mg vials:

Reconstituting a 2 mg vial to create a 1 mg/mL concentration:

To create a 1 mg/mL concentration with the initial 2 mg of powder medication, you can use the following formula:

So, you would need to add 2 mL of bacteriostatic water to the vial containing 2 mg of powder medication to achieve a concentration of 1 mg/mL.

Reconstituting a 5 mg vial to create a 2 mg/mL concentration:

To create a 2.4 mg/mL concentration with 5 mg of powder medication, you can use the same formula:

So, you would need to add ~2.08 mL of bacteriostatic water to the vial containing 5 mg of powder medication to achieve a concentration of 2.4 mg/mL.

Semaglutide Doses

For your research, you may need to recreate the same doses that were involved in the initial STEP trials. Below we summarize tables with some of the options for reconstituting 2 mg and 5 mg vials to obtain the doses of semaglutide approved for chronic weight management.

Reconstitution of 2 mg vial

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Dose	Volume of bacteriostatic water (mL)*	Volume of solution in the syringe (mL)**	Number of doses in one vial***	Concentration per 1 mL
0.25 mg	2 mL	0.25 mL	8	1 mg/mL
0.5 mg	2 mL	0.5 mL	4	1 mg/mL
1 mg	1 mL	0.5 mL	2	2 mg/mL
1.7 mg	1 mL	1 mL	1	2 mg/mL

Chart Tips

*This column tells you how much bacteriostatic water you should add to the vial.
**This column tells you how much of the produced solution you should draw up in the syringe so that the syringe contains the desired dose.
***This column tells how many times you can draw up the desired dose with the syringe per vial.

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Reconstitution of 5 mg vial

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Dose	Volume of bacteriostatic water (mL)*	Volume of solution in the syringe (mL)**	Number of doses in one vial***	Concentration per 1 mL
0.5 mg	2 mL	0.2 mL	10	2.5 mg/mL
1 mg	1 mL	0.2 mL	5	5 mg/mL
1.7 mg	1 mL	0.33 mL	3	5 mg/mL
2.4 mg	1 mL	0.5 mL	2	5 mg/mL

Chart Tips

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Semaglutide Mixing Instructions

Uncover the rubber stopper by removing the plastic cap from the semaglutide vial

Uncover the rubber stopper by removing the plastic cap from the bacteriostatic water vial

Safeguard against bacterial contamination by swabbing the rubber stoppers with alcohol pads

Insert the needle of the syringe into the rubber stopper of the bacteriostatic water vial

Withdraw the desired amount of bacteriostatic water from the vial by slowly pulling back the plunger to the corresponding mark on the syringe

Once the bacteriostatic water is in the syringe, remove the needle from the vial of the bacteriostatic water and insert it into the rubber stopper of the semaglutide vial

Add bacteriostatic water into the semaglutide vial gradually by injecting

Swirl the solution gently until complete dissolution of the peptide. Avoid shaking the vial

How to Store Reconstituted Semaglutide

Temperature

Store peptides reconstituted with solvent in the refrigerator, typically at temperatures ranging from 2°C to 8°C.

Aliquoting

Consider dividing the reconstituted semaglutide solution into smaller aliquots. This reduces the risk of degradation.

Labeling

Clearly label the containers with the date of reconstitution and the concentration of the solution. This helps in tracking the age and potency of the solution.

Do Not Freeze

Do not freeze semaglutide reconstituted semaglutide as this will cause the product to lose its properties and effectiveness.

Learn More About Semaglutide

Have questions about semaglutide?
Go to our Semaglutide FAQ to learn more, or contact us.

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